Coding

Part:BBa_K4812000

Designed by: Rashik Chand   Group: iGEM23_NYU-Abu-Dhabi   (2023-10-07)


Scl1.1

Sequence of collagen-like protein from Streptococcus pyogenes. GenBank: AF252861.1

Usage and Biology

Scl 1.1 is related to Scl2.28, but each has a significantly different primary amino acid sequence. Scl1.1 is made of Ss, signal sequence; V, variable region; CL, collagen-like region; L, linker region; and WM, cell wall/membrane region. The collagen-like region in Scl1.1 is made of 50 Gly-Xaa-Yaa (GXY) motifs that lead to the triple helical structure. The N-terminus of the protein is called the variable (V) region, composed of a noncollagenous sequence and consists of 67 amino acids in Scl1.1. Scl1 forms a lollipop-like structure with the stalk being made of collagen-like region and globular head made of the V region.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 453
    Illegal SpeI site found at 183
    Illegal PstI site found at 995
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 183
    Illegal PstI site found at 995
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 453
    Illegal SpeI site found at 183
    Illegal PstI site found at 995
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 453
    Illegal SpeI site found at 183
    Illegal PstI site found at 995
    Illegal AgeI site found at 643
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 485
    Illegal BsaI.rc site found at 1211


[edit]
Categories
Parameters
None